The preferred, non-invasive method is the measurement of breath hydrogen content.  In this method, breath samples are collected as you exhale. The exhaled gases are analyzed for hydrogen, a byproduct of bacteria that breakdown the lactose that is not absorbed. 

If this test is not available, blood samples can be taken at various time to measure the amount of glucose, a product of lactose breakdown, in your blood. These blood samples are taken immediately prior to the test and then at various times after drinking the lactose solution (usually 30, 60, and 120 minutes).

Blood collection (Adult or child):
Blood is drawn from a vein (venipuncture), usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic, and a tourniquet (an elastic band) or blood pressure cuff is placed around the upper arm to apply pressure and restrict blood flow through the vein. This causes veins below the tourniquet to distend (fill with blood). A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the tourniquet is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

Blood collection (Infant or young child):
The area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. Cotton or a bandage may be applied to the puncture site if there is any continued bleeding.

How to Prepare for the Test

Fast for 8 hours and avoid strenuous exercise for 8 hours before the test.

Infants and children:
The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experience, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:

• infant test or procedure preparation (birth to 1 year)
• toddler test or procedure preparation (1 to 3 years)
• preschooler test or procedure preparation (3 to 6 years)
• schoolage test or procedure preparation (6 to 12 years)
• adolescent test or procedure preparation (12 to 18 years)

How the Test will Feel

The sampling of an exhaled breath is conveniently collected in either a special bag or syringe, and should not be associated with any pain or discomfort.

When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.

Why the Test is Performed

This test is used to diagnose lactase deficiency.

Lactose is a disaccharide (a type of sugar) in dairy products. During digestion, lactose is broken down into glucose and galactose, two simple sugars, by the intestinal enzyme lactase. Lactose-intolerant patients have a deficient or absent lactase enzyme. Bacteria metabolize the undigested lactose, producing gas such as hydrogen and other acid products.

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