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Sputum DFA (direct fluorescent antibody)   

Overview | Risks | Results

Alternative names:

Direct immunofluoresence test

Definition:

A test performed to detect the presence of microorganisms in sputum by using antibodies tagged with a fluorescent dye.

How the Test is Performed

A sputum sample is obtained by coughing into a specimen container. In the laboratory, antibodies that have been chemically linked to a fluorescent dye are added to the sample. The "flagged" antibodies will bind to the specific antigens (in this case, the microorganism against which they were formed). The specific microorganism (antigen) is indirectly identified by the presence of fluorescence when examined under a special microscope.

How to Prepare for the Test

Obtain a coughed sputum specimen. If there is not a productive cough, a respiratory treatment may produce a sample.

How the Test will Feel

There is no discomfort.

Why the Test is Performed

This test may be performed when certain types of pulmonary infections or pneumonias are suspected.

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